Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein
Identifieur interne : 001494 ( Main/Exploration ); précédent : 001493; suivant : 001495Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein
Auteurs : Yong-Sheng Wang ; Chung-Ke Chang ; Ming-Hon HouSource :
- Acta Crystallographica Section F [ 2053-230X ] ; 2015-08-01.
Abstract
The N‐terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS‐CoV NP‐NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. In this study, the crystallization and crystallographic analysis of MERS‐CoV NP‐NTD (amino acids 39–165), with a molecular weight of 14.7 kDa, are reported. MERS‐CoV NP‐NTD was crystallized at 293 K using PEG 3350 as a precipitant and a 94.5% complete native data set was collected from a cooled crystal at 77 K to 2.63 Å resolution with an overall Rmerge of 9.6%. The crystals were monoclinic and belonged to space group P21, with unit‐cell parameters a = 35.60, b = 109.64, c = 91.99 Å, β = 101.22°. The asymmetric unit contained four MERS‐CoV NP‐NTD molecules.
Url:
DOI: 10.1107/S2053230X15010146
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000969
- to stream Istex, to step Curation: 000969
- to stream Istex, to step Checkpoint: 000205
- to stream Main, to step Merge: 001496
- to stream Main, to step Curation: 001494
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein</title><author><name sortKey="Wang, Yong Heng" sort="Wang, Yong Heng" uniqKey="Wang Y" first="Yong-Sheng" last="Wang">Yong-Sheng Wang</name></author><author><name sortKey="Chang, Chung E" sort="Chang, Chung E" uniqKey="Chang C" first="Chung-Ke" last="Chang">Chung-Ke Chang</name></author><author><name sortKey="Hou, Ming On" sort="Hou, Ming On" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C3B5F5FB070CC05A15D0F4CB89100C34C7F2B621</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1107/S2053230X15010146</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-B7DGJ9PD-W/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000969</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000969</idno><idno type="wicri:Area/Istex/Curation">000969</idno><idno type="wicri:Area/Istex/Checkpoint">000205</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000205</idno><idno type="wicri:doubleKey">2053-230X:2015:Wang Y:crystallographic:analysis:of</idno><idno type="wicri:Area/Main/Merge">001496</idno><idno type="wicri:Area/Main/Curation">001494</idno><idno type="wicri:Area/Main/Exploration">001494</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein</title><author><name sortKey="Wang, Yong Heng" sort="Wang, Yong Heng" uniqKey="Wang Y" first="Yong-Sheng" last="Wang">Yong-Sheng Wang</name></author><author><name sortKey="Chang, Chung E" sort="Chang, Chung E" uniqKey="Chang C" first="Chung-Ke" last="Chang">Chung-Ke Chang</name></author><author><name sortKey="Hou, Ming On" sort="Hou, Ming On" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name></author></analytic><monogr></monogr><series><title level="j" type="main">Acta Crystallographica Section F</title><title level="j" type="alt">ACTA CRYSTALLOGRAPHICA SECTION F</title><idno type="ISSN">2053-230X</idno><idno type="eISSN">2053-230X</idno><imprint><biblScope unit="vol">71</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="977">977</biblScope><biblScope unit="page" to="980">980</biblScope><publisher>International Union of Crystallography</publisher><pubPlace>5 Abbey Square, Chester, Cheshire CH1 2HU, England</pubPlace><date type="published" when="2015-08-01">2015-08-01</date></imprint><idno type="ISSN">2053-230X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">2053-230X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The N‐terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS‐CoV NP‐NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. In this study, the crystallization and crystallographic analysis of MERS‐CoV NP‐NTD (amino acids 39–165), with a molecular weight of 14.7 kDa, are reported. MERS‐CoV NP‐NTD was crystallized at 293 K using PEG 3350 as a precipitant and a 94.5% complete native data set was collected from a cooled crystal at 77 K to 2.63 Å resolution with an overall Rmerge of 9.6%. The crystals were monoclinic and belonged to space group P21, with unit‐cell parameters a = 35.60, b = 109.64, c = 91.99 Å, β = 101.22°. The asymmetric unit contained four MERS‐CoV NP‐NTD molecules.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chang, Chung E" sort="Chang, Chung E" uniqKey="Chang C" first="Chung-Ke" last="Chang">Chung-Ke Chang</name><name sortKey="Hou, Ming On" sort="Hou, Ming On" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name><name sortKey="Wang, Yong Heng" sort="Wang, Yong Heng" uniqKey="Wang Y" first="Yong-Sheng" last="Wang">Yong-Sheng Wang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001494 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001494 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:C3B5F5FB070CC05A15D0F4CB89100C34C7F2B621
|texte= Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein
}}
| This area was generated with Dilib version V0.6.33. |  |